HiDef-B8 Stem Cell Growth Medium
Weekend-Free Stem Cell Maintenance Made Easy
HiDef-B8 is a complete, serum-free medium specifically designed for feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state.
HiDef-B8 is available in two flexible formats:
Stem Cell Maintenance Kit:
Includes the 400X HiDef B8 Supplement vial and DMEM/F12 basal medium, highly stable.
HiDef-B8 400X Supplement Only:
Ideal for labs preferring their own basal medium.
Why Choose HiDef-B8? Achieve More
Serum-Free, Feeder-Free
Mantain PSCs in an undifferentiated state with a defined xeno-free formulation
Weekend-Free Maintenance
Flexible feeding schedules save you time and resources
Customizable Applications
Choose your preferred matrices and passaging methods
Proven Performance
Long-term maintenance of PSCs with consistent results
Key Ingredients for Optimal Performance
HiDef-B8 contains essential components required for PSC maintenance (Insulin, Ascorbic Acid-2-Phosphate, Transferrin, Sodium Selenite, TGFB3, NRG1, Thermostable FGF2/bFGF).
No additional supplements are needed.
Proven Effectiveness in Stem Cell Culture
HiDef-B8 has been rigorously tested and validated:
Long-Term Maintenance:
- Our standard hiPSC line 19c3 has been cultured for 100+ passages, maintaining expression of key markers for undifferentiated status SSEA4 and TRA-1-60 (validated via flow cytometry).
- PSCs exhibit hESC-like morphology and normal karyotype.
Patient-Derived iPSCs:
- HiDef-B8 has been used to generate hiPSC lines from 37 patients using established protocols, all maintaining expression of SSEA4 and TRA-1-60 in culture (up to passage 65).
- Cultured in HiDef-B8, hiPSCs maintained hESC-like morphology and positive immunofluorescent staining for SSEA4, POU5F1, SOX2, and TRA-1-60; and showed a stable karyotype in long-term culture
Flow cytometry analysis showing the expression of undifferentiated markers in hiPSC line 19c3 cultured in B8 medium from passage (p) 21 to p131 (left), and in 37 individual hiPSC lines derived in B8 from p12 to p65 (right).
Phase-contrast images of hiPSC line 19c3 p44 cultured in B8. Scale bar, 100 mm
Streamline Your Stem Cell Workflow
Experience the benefits of HiDef-B8 for your research
Save Time: Weekend-free maintenance eliminates unnecessary feeding schedules
Flexible Formats: Tailor HiDef-B8 to your workflow with our kit or supplement options
Reliable Results: Achieve consistent, long-term maintenance of undifferentiated PSCs
Optimize your stem cell research today with HiDef-B8!
Downloads
FGF2-G3 (bovine) Growth Factor
User Guide
FGF2 (chicken) Growth Factor
User Guide
DMEM/F12 Basal Medium
User Guide
FGF2 Screening Kit Growth Factors
User Guide
HiDef-B8 Stem cell maintenance medium
User Guide
HiDef-ITS Serum replacement supplement
User Guide
FGF2 Product Info Guide
User Guide
Ready-CEPT Cell Viability Cocktail USER
User Guide
FGF2 (tuna) Growth Factor
User Guide
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