The EZdetectTM Hoechst stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain Hoechst by cellular DNA. It selectively binds minor grooves of the DNA and hence allows for efficient staining of the nuclei. Hoechst stain can be excited either with a xenon mercury arc lamp or a UV laser and is detected through a blue filter. Mycoplasma staining with Hoechst appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

Kühlware (2°C - 8°C)

Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. This kit has been designed for detection of mycoplasma based on amplification of spacer region between 16S and 23S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. This allows for detection of commonly encountered cell culture contaminants. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 350-400bp derived from the internal control DNA on the agarose gel.

Versand auf Trockeneis (-20 °C).

The EZcountTM XTT Cell Assay kit is designed for determination of cell viability and cell proliferation and / or effect of cytotoxic agent. This kit is based on quantitative measurement of extracellular reduction of XTT to water soluble orange coloured formazan derivative by metabolically active cells.

Versand auf Trockeneis (-20 °C).

Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. PCR based detection of mycoplasma is one of the most convenient method. This kit has been designed for detection of mycoplasma based on amplification of 16S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 502 - 520bp derived from the internal control DNA on the agarose gel.

Versand auf Trockeneis (-20 °C).

Melondialdehyde(MDA) is one of such TBARS used for estimation of lipid peroxidation. This assay is based on the reaction of MDA with a chromogenic agent thiobarbituric acid(TBA) at high temperature and acidic conditions to form MDA-TBA adduct(1:2). This complex has absorption maximum at 532nm and can be measured spectrophotometrically between 530 - 540nm. Absorbance is directly proportional to the TBARS concentration in the sample.

Kühlware (2°C - 8°C)

EZAssayTM GST Activity Estimation kit is simple and convenient tool for sensitive and reproducible detection and quantitation of GST activity in an array of samples like serum, cell lysates and tissue homogenates. This assay is based on the GST-catalyzed reaction between reduced glutathione and the GST substrate 1-chloro-2,4-dinitrobeneze(CDNB). CDNB can react with the broadest range of GST isoenzymes. The conjugation of glutathione to the CDNB substrate results in an increase in absorbance at 340nm.

Versand auf Trockeneis (-20 °C).

The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.

Versand auf Trockeneis (-20 °C).

The EZcountTM WST-1 Cell Assay kit is designed for determination of cell viability, cell proliferation and cytotoxicity. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye WST-1 [2-(4-Iodophenyl)-3-4 -nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to a water-soluble coloured formazan product by metabolically active cells.

Versand auf Trockeneis (-20 °C).

The EZcountTM Neutral Red Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of uptake of neutral red by cellular lysosomes. Neutral red is a weak cationic dye that readily penetrates the cell membrane by non-ionic diffusion.

Alkaline phosphatase(ALP) is a group of isoenzymes that hydrolyze phosphate esters and generate inorganic phosphate at an alkaline pH. CCK035 is a simple colorimetric kit designed to detect the presenceof alkaline phosphatase activity.

Kühlware (2°C - 8°C)

Lactate dehydrogenase(LDH) is a cytoplasmic enzyme present in all mammalian cells. Plasma membrane of live cells is impermeable to LDH. However it is rapidly released into the culture medium when plasma membrane is damaged. CCK036 has been designed for rapid determination of cell cytotoxicity using an enzyme coupled two step reaction.

Versand auf Trockeneis (-20 °C).

L-glutamate is most abundant intracellular amino acid whereas L-glutamine is the most abundant extracellular amino acid. Estimation of L-glutamate and L-glutamine levels in the culture medium is thus indicative of the cellular metabolism and health of the cells. CCK037 is designed for estimation of concentration of these amino acids in cell culture samples such as cell lysates and spent media.

Versand auf Trockeneis (-20 °C).

Microscopy Slide Set for Mammalian Cell lines
Contains a set of slides of five cell lines:
a) BHK-21
b) CHO
c) Vero
d) HEK-293
e) Sp2/O

The EZcountTM ATP Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. The kit is based on the generation of luminescence as ATP catalyzes the reaction between D-luciferin and enzyme luciferase. ATP is ubiquitously present in all viable, metabolically active cells. On the other hand, cell death is characterized by a significant decrease in the ATP level.

Versand auf Trockeneis (-20 °C).

The EZcount™ Sulphorhodamine B cell assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. This kit is based on the quantitative measurement of cellular protein.

The EZcountTM MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

Versand auf Trockeneis (-20 °C).

CCK058 has been designed for rapid determination of cell cytotoxicity in a one step reaction. Non-toxicity of the LDH reagent allows performing the assay in a single culture vessel i.e. in homogenous format. The reagent can accurately determine cell cytotoxicity in the samples containing mixed population of viable and dead cells. This feature makes the method suitable for high throughput automation wherein minimum handling and manipulations are required.

Versand auf Trockeneis (-20 °C).

It is based on the reduction of nitrate(NO3-) to nitrite(NO2-) by a reducing agent at 37°C. Converted nitrite and endogenous nitrite are collectively converted by Griess reagent to a blue colored azo compound. This compound can be measured spectrophotometrically between 580-630nm and absorbance is directly proportional to the total nitric oxide concentration in the sample which is calculated from standard plot of NaNO3.

Kühlware (2°C - 8°C)

EZdetectTM Cell Senescence Detection Kit is designed for detection of cell senescence in culture. This kit is based on the qualitative detection of senescent cells that exhibit increased activity of acidic β-galactosidase, termed senescence-associated β-galactosidase(SA-β-gal). SA-β-gal is the most widely accepted marker of senescent cells

Versand auf Trockeneis (-20 °C).
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Glucos-6-Phosphate Dehydrogenase(G6PD) is a cytosolic enzyme present in all cells including RBCs. Plasma membrane of live cells is impermeable to G6PD. However, it is rapidly released into the culture medium when plasma membrane is damaged. The quantity of the released G6PD is used as a measure of cell cytotoxicity. CCK069 has been designed for rapid determination of cell death. The dye used in the assay is non-radioactive, water soluble and stable in most of the culture media.

Versand auf Trockeneis (-20 °C).