Real-Time Cell Analysis:

Highly sensitive, non-invasive, easy to operate


xCELLigence Real-Time Cell Analysis (RTCA) - Continuously track cell health, behaviour, and function with label-free impedance and imaging assays. 

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Key Fa​​​​cts

System​​​​s

A​​​​pplications

Technology

Consumables

Literature

Challenges of Cell-Based Assays using Traditional Methods

Time-consuming and labour-intensive 

Poor reproducibility and high variability

Low sensitivity and delayed detection

Need for labels, dyes or stainings

Only end-point data available

Solved by xCELLigence RTCA

  • Real-time Monitoring
  • Label-free and non-invasive
  • High Sensitivity and Accuracy
  • Scalable High-Throughput Options
  • Reproducible results
  • Easy to use

Revolutionizing Cell-Based Research Real-Time, Live Cell Analysis​

Experience unparalleled insight into cellular processes in real-time, under pysiological conditions.

Detect and quantify apoptosis with precision using xCELLigence RTCA eSight. 

Straightforward analyis of barrier integrity and cytotoxicity using xCELLigence RTCA.

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Real-Time Monitoring

Gain continuous, dynamic insights into cellular processes, including proliferation, cytotoxicity, barrier function, migration, invasion, and immune cell-mediated killing.

Non-Invasive Technology

Neither the gold microelectrode surfaces nor the applied electric potential (22 mV) have an effect on cell health or behavior.  

Simple and User-Friendly

The easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time. 

Label Free & Physiologically Relevant

Preserve natural cell behavior with non-destructive, dye-free analysis, enabling long-term observation while reducing workload.

Unmatched Sensitivity

   Detect subtle cellular changes, even at low effector-to-target ratios, with kinetic data that reveal dynamic cell activities. 

Short- and Long-Term Flexibility

  Monitor cellular responses over seconds or days to capture a complete kinetic profile of behavior.  

xCELLigence RTCA Analyzers

Tailored Solutions for Your Research Needs and Throughput Requirements

 

xcelligence rtca mp

xCELLigence RTCA MP - Multiple Plates

Perform real-time immune cell killing assays in up to 6x96 well-plates.

xcelligence rtca esight

xCELLigence RTCA eSight - Imaging & Impedance

Combine impedance-based measurements with live cell imaging.

xcelligence rtca dp

xCELLigence RTCA DP - Cell Invasion & Migration

Analyze cell migration and invasion with precision and ease in up to 48 wells.

xcelligence rtca s16

xCELLigence RTCA S16 - Pilot Scale

Continuously monitor cell proliferation, cytopathic effects and morphological changes in a 16-well format. 

xcelligence rtca single plate

xCELLigence RTCA SP - Single Plate

Continously monitor cell proliferation, cytopathic effects, viral titers and morphological changes in a 96-well format.

xcelligence rtca high throughput

xCELLigence RTCA HT - High Throughput Model

Monitor up to four 384-well plates in real time to meet high throuput screening needs.

xcelligence rtca cardio

xCELLigence RTCA Cardio - Cardiomyocyte Contractility

Monitor cardiomyocyte contractility and viability in real time.

xcelligence rtca cardio ECR

xCELLigence RTCA CardioECR - Cardiomyocyte Contractility & Electrical Activity

Simultaneously analyse cardiomyocyte contractility, viability, and electrophysiology

xcelligence rtca ePACER

xCELLigence RTCA ePACER- Cardiomyocate Pacing

Easy solution for efficient maturation of hiPSC cardiomyocytes with less stress

Download Overview: xCELLigence RTCA Systems

 Applications

 

Immune Cell Killing 

Cytotoxicity Monitoring 

Cell Adhesion Analysis  

Apoptosis and Proliferation

Cell Invasion and Migration

Stem Cells  

Cell Barrier Function  

Cell Signaling Monitoring

Viral Cytopathic Effects  

Phagocytosis

Cardio Safety Toxicology 

Cardiac Disease Modeling

Cardio Drug Discovery

Cardiomyocyte Maturation​ 

 The CiPA (Comprehensive in Vitro Proarrhythmig Assay) Initiative 

Technology - Cellular Impedance Explained​

Label-free, real-time method for studying cells  

Cellular impedance is a fast, non-invasive method for real-time live-cell analysiswithout the need for labels or staining. Unlike traditional assays, it continuously monitors cell behavior by measuring electrical impedance changes as cells interact with electrodes in the plate bottom.

With exceptional speed and temporal resolution, this technology captures key cell metrics—proliferation, adhesion, morphology, migration, and differentiation—over seconds to days. By eliminating endpoint limitations, it delivers dynamic, quantitative insights for more accurate and comprehensive cell analysis.

Learn M​​​​ore 

Consumables

xcelligence rtca microplates

RTCA  Microplates, Reagents & Kit

Literature

Handbooks

Cancer Immunotherapy

Evaluating Immune Cell Therapy Potency

Vaccine and Virology Applications

Infectious Disease Applications

Case Studies 

Building a CAR-T Toolbox for More Comprehensive Assessment of Cell Therapies

Roundup: Quantifying Immune‑Cell Mediated Killing

FAQ

Your questions answered

xCELLigence is a real-time cell analysis (RTCA) platform that uses label-free impedance monitoring to continuously track cell viability, proliferation, morphology, and function without the need for dyes or stains.

The system measures electrical impedance across a microelectrode-integrated well plate (E-Plate®). As cells grow, attach, or die, impedance changes are detected and translated into quantitative, real-time data on cell behavior.

✔ Real-time, continuous monitoring instead of endpoint measurements

✔ Label-free analysis preserves cell viability and avoids artifacts

✔ High sensitivity detects small changes in cell adhesion, proliferation, and apoptosis

✔ Automated, hands-free data acquisition improves reproducibility

✔ Multiparametric insights for a deeper understanding of cell health

xCELLigence is widely used in drug discovery, immunotherapy, cancer research, and toxicology, supporting assays such as:

  • Cytotoxicity & drug screening
  • Cell proliferation & viability
  • Apoptosis & cell death analysis
  • Immune cell-mediated killing
  • Cell adhesion & migration studies
  • Barrier function & permeability assays


Unlike MTT, Trypan Blue, or flow cytometry, which require staining, cell lysis, or fixation, impedance monitoring is non-invasive and provides continuous, real-time data on live cells. This results in more physiologically relevant and reproducible data.  





No! xCELLigence systems feature intuitive software for automated data acquisition and analysis. No specialized training is needed, making it accessible for both experienced and new researchers.





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