Apoptosis and Proliferation Assays


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xCELLigence RTCA eSight - Imaging & Impendance

The Agilent xCELLigence RTCA eSight is an advanced real-time cell analysis system that enables comprehensive insight into a cell's health, behavior, function, and biological processes using live, simultaneous, and real-time biosensor-based and image-based measurements. The eSight system combines label-free impedance technology with brightfield imaging, three fluorescent colors (red, green, and blue), and 5x, 10x, and 20x objectives. Boost your confidence and get more insightful data with ease as you maximize your efficiency with five total readouts.

Features

  • Combine label-free impedance-based xCELLigence Real-Time Cell Analysis technology with live-cell imaging in red, green, and blue spanning 5, 10, and 20x objectives for increased confidence in your cell analysis and conclusion
  • Whole well imaging expansion: Visualize the entire well when doing QC or quantifying and monitoring cell growth
  • Multiplexed and information-rich assays combining cellular impedance and imaging in the same well, all inside a culture incubator
  • Deliver physiologically relevant data whether 2D or 3D based cell cultures, with a simple workflow that adapts to differing experimental protocols while monitoring cell health, behavior, and function
  • Utilize flexible live-cell imaging brightfield capabilities and three fluorescent channels spanning a multitude of microplate forms (6-, 12-, 24-, 48-, 96-, or 384- well) and vessel types (35- or 60-mm dishes), and the capability of user-defined schedules
  • Analyze a continuous readout of cell number, proliferation, cell size/shape, changes to morphology, and cell-substrate attachment quality 

How It Works

Step 1

Seed cells, then place plate or tissue culture vessels on the eSight in the incubator.  Set up data and imaging acquisition schedule using eSight software.

Step 2

Add treatments (e.g. immune effector cells, antibodies, anti-viral drugs.)

Step 3

Automatically acquire data with the proprietary xCELLigence biosensor technology and overlay images in real time.

culture vessels on esight


expanded imaging capabilities

The xCELLigence RTCA eSight delivers expanded imaging capabilities for easy multiplexing. Its unique optical hardware offers brightfield, red, green, and blue fluorescent channels with 5, 10, or 20x magnification, all in the same well; and supports a multitude of microplate formats.