xCELLigence RTCA — Real-Time Cell Analysis
Highly sensitive, non-invasive, easy to operate
xCELLigence Real-Time Cell Analysis (RTCA) - Continuously track cell health, behaviour, and function with label-free impedance and imaging assays.
Challenges of Cell-Based Assays using Traditional Methods
Time-consuming and labour-intensive
Poor reproducibility and high variability
Low sensitivity and delayed detection
The need for labels, dyes or stainings
Only end-point data available
Solved by xCELLigence RTCA
- Real-time monitoring
- Label-free and non-invasive
- High sensitivity and accuracy
- Scalable high-throughput options
- Reproducible results
- Easy to use
Revolutionizing Cell-Based Research:
Real-Time, Live Cell Analysis
Experience unparalleled insight into cellular processes in real-time, under physiological conditions.
Detect and quantify apoptosis using xCELLigence RTCA eSight.
Straightforward analysis of barrier integrity and cytotoxicity using xCELLigence RTCA.
Real-Time Monitoring
Gain
continuous, dynamic insights into cellular processes, including proliferation,
cytotoxicity, barrier function, migration, invasion, and immune cell-mediated
killing.
Non-Invasive Technology
Neither the gold microelectrode surfaces nor the applied electric potential (22 mV) have an effect on cell health or behavior.
Simple and User-Friendly
The easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time.
Label Free & Physiologically Relevant
Preserve natural cell behavior with non-destructive, dye-free analysis, enabling long-term observation while reducing workload.
Unmatched Sensitivity
Detect subtle cellular changes, even at low
effector-to-target ratios, with kinetic data revealing dynamic cellular changes.
Short- and Long-Term Flexibility
Monitor
cellular responses over seconds or days to capture a complete kinetic profile
of cell behavior.
xCELLigence RTCA Analyzers
Tailored solutions for your research needs and throughput requirements.
xCELLigence RTCA MP - Multiple Plates
Perform real-time immune cell killing assays in up to 6x96 well-plates.
xCELLigence RTCA eSight - Imaging & Impedance
Combine impedance-based measurements with live cell imaging.
xCELLigence RTCA DP - Invasion & Migration
Analyze cell migration and invasion with
precision and ease in up to 48 wells.
xCELLigence RTCA S16 - Pilot Scale
Continuously monitor cell proliferation, cytopathic effects and morphological changes in a 16-well format.
xCELLigence RTCA SP - Single Plate
Continuously monitor cell proliferation, cytopathic effects, viral titers and morphological changes in a 96-well format.
xCELLigence RTCA HT - High Throughput
Monitor up to four 384-well plates in real time to meet high throughput screening needs.
xCELLigence RTCA Cardio - Cardiomyocyte Contractility
Monitor cardiomyocyte contractility and viability in real time.
xCELLigence RTCA CardioECR - Cardiomyocyte Contractility & Electrical Activity
Simultaneously analyse cardiomyocyte contractility, viability, and electrophysiology.
xCELLigence RTCA ePACER – Cardiomyocyte Pacing
Easy solution for efficient maturation of hiPSC cardiomyocytes with less stress.
Applications
Technology -
Cellular Impedance Explained
Label-free, real-time method for studying cells.
Cellular impedance is a fast, non-invasive method for real-time live-cell analysis without the need for labels or staining. Unlike traditional assays, it continuously monitors cell behavior by measuring electrical impedance changes as cells interact with electrodes in the plate bottom.
With exceptional speed and temporal resolution, this technology captures key cell metrics, such as proliferation, adhesion, morphology, migration, and differentiation, over seconds to days. By eliminating endpoint limitations, it delivers dynamic, quantitative insights for more accurate and comprehensive cell analysis.
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Consumables
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FAQ
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