Real-Time Cell Analysis:
Highly sensitive, non-invasive, easy to operate
xCELLigence Real-Time Cell Analysis (RTCA) - Continuously track cell health, behaviour, and function with label-free impedance and imaging assays.
Challenges using Traditional Methods
Time-consuming and labour-intensive
Poor reproducibility and high variability
Low sensitivity and delayed detection
The need for labels, dyes or stainings
Only end-point data available
Solved by xCELLigence RTCA
- Real-time Monitoring
- Label-free and non-invasive
- High Sensitivity and Accuracy
- Scalable High-Throughput Options
- Reliable anDetect and quantify apoptosis with precision using xCELLigence RTCA eSight. d Reproducible Results
- Easy to use
Detect and quantify apoptosis with precision using xCELLigence RTCA eSight.
Straightforward analyis of barrier integrity and cytotoxicity using xCELLigence RTCA.
Ap.Prof. Julia Eckl-Dorna and Aldine Tu, MSc Medical University of Vienna
Technology -
Cellular Impedance Explained
Label-free, real-time method for studying cells.
Cellular impedance is a fast, non-invasive method for real-time live-cell analysis without the need for labels or staining. Unlike traditional assays, it continuously monitors cell behavior by measuring electrical impedance changes as cells interact with electrodes in the plate bottom.
With exceptional speed and temporal resolution, this technology captures key cell metrics, such as proliferation, adhesion, morphology, migration, and differentiation, over seconds to days. By eliminating endpoint limitations, it delivers dynamic, quantitative insights for more accurate and comprehensive cell analysis.
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Revolutionizing Cell-Based Research:
Real-Time, Live Cell Analysis
Experience unparalleled insight into cellular processes in real-time, under physiological conditions.
Real-Time Monitoring
Gain
continuous, dynamic insights into cellular processes, including proliferation,
cytotoxicity, barrier function, migration, invasion, and immune cell-mediated
killing.
Non-Invasive Technology
Neither the gold microelectrode surfaces nor the applied electric potential (22 mV) have an effect on cell health or behavior.
Simple and User-Friendly
The easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time.
Label Free & Physiologically Relevant
Preserve natural cell behavior with non-destructive, dye-free analysis, enabling long-term observation while reducing workload.
Unmatched Sensitivity
Detect subtle cellular changes, even at low
effector-to-target ratios, with kinetic data that reveal dynamic cell
activities.
Short- and Long-Term Flexibility
Monitor
cellular responses over seconds or days to capture a complete kinetic profile
of behavior.
xCELLigence RTCA Analyzers
Tailored solutions for your research needs and throughput requirements.
xCELLigence RTCA MP - Multiple Plates
Perform real-time immune cell killing assays in up to 6x96 well-plates.
xCELLigence RTCA eSight - Imaging & Impedance
Combine impedance-based measurements with live cell imaging.
xCELLigence RTCA DP - Cell Invasion & Migration
Analyze cell migration and invasion with
precision and ease in up to 48 wells.
xCELLigence RTCA S16 - Pilot Scale
Continuously monitor cell proliferation, cytopatic effects and morphological changes in a 16-well format.
xCELLigence RTCA SP - Single Plate
Continously monitor cell proliferation, cytopatic effects, viral titers and morphological changes in a 96-well format.
xCELLigence RTCA HT - High Throughput Model
Monitor up to four 384-well plates in real time to meet high throuput screening needs.
xCELLigence RTCA Cardio - Cardiomyocyte Contractility
Monitor cardiomyocyte contractility and viability in real time.
xCELLigence RTCA CardioECR - Cardiomyocyte Contractility & Electrical Activity
Simultaneously analyse cardiomyocyte contractility, viability, and electrophysiology.
xCELLigence RTCA ePACER- Cardiomyocate Pacing
Easy solution for efficient maturation of hiPSC cardiomyocytes with less stress.
Consumables
Applications
Label-free, real-time monitoring of immune cell killing with high sensitivity and reproducibility. Capture killing potency, serial killing, and exhaustion in a simple, high-throughput workflow that closely mimics in vivo activity.
Track drug or compound effects on cell health, proliferation, and morphology in real time. Generate dose-response curves and monitor long-term cellular responses with minimal effort.
Monitor cell attachment and spreading continuously to study metastasis, without labels or dyes.
Apoptosis and Proliferation Assays
Real-time, high-throughput monitoring of cell apoptosis and proliferation. Easily capture and quantify dynamic changes in adhesion, viability, and cell health without labels.
Cell Invasion and Migration Assays
Effortlessly track cell migration and invasion in real time with xCELLigence RTCA—fully automated, label-free, and quantitative.
Track stem cell growth, attachment, and morphology in real time to ensure consistency and functionality
between passages.
Detect disruption in endothelial and epithelial barrier intergity in real time using xCELLigence RTCA, a sensitive, label-free alternative to TEER and permeability assays.
Capture dynamic, label-free changes in cell size, shape, and proliferation in response to signaling events, such as GPCR, RTK, and nuclear hormones - from minutes to days.
Viral Cytopathic Effect Assays
Track viral CPE automatically and in real time, eliminating labor-intensive plaque assays. Measure infection kinetics, cytopathogenicity, and optimal time points in a single, streamlined workflow.
Phagocytosis Assays
Monitor phagocytosis in real time: Track immune cell activity, visualize macrophages, and uncover potential therapeutic targets with dynamic, high-content analysis.
The CiPA (Comprehensive in Vitro Proarrhythmig Assay) Initiative
The CiPA Initiative, led by HESI, FDA, and the Safety Pharmacology Society, is redefining cardiac safety testing. The xCELLigence RTCA CardioECR plays a role in both, Phase I and II, of this initiative.
Cardiac Disease Modeling
Study heart disease at the cellular level using hiPSC-derived cardiomyocytes to model genetic cardiac disorders and uncover mechanisms of electrical and contractile dysfunction.
Cardio Safety Toxicology
Assess drug-induced cardiac toxicity with hiPSC-derived cardiomyocytes using xCELLigence RTCA CardioECR for real-time, multiplexed insights into compound safety.
Cardio Drug Discovery
Accelerate cardiac drug discovery using hiPSC-derived cardiomyocytes to evaluate efficacy and toxicity, including real-time insights into compound effects on heart cell contractility.
Cardio Maturation
Enhance hiPSC-cardiomyocyte maturation with long-term electrical pacing for improved gene expression, protein function, and contractility - ideal for drug testing, discovery, and disease modeling
FAQ
Your questions answered