Traditional cell culture is limited to 2D growth on the XY plane, unlike the natural 3D environment of living organisms. In vivo, cells exist in complex 3D microenvironments with organized cell-cell and cell-matrix interactions and dynamic nutrient transport. This makes standard 2D cell culture less reliable for modeling in vivo drug efficacy and toxicity—key factors in drug discovery.
3D spheroids more accurately mimic in vivo tissues by replicating cellular communication and the formation of extracellular matrices (ECMs). These ECMs allow cells to move within the spheroid, similar to their behavior in living tissue, making spheroids better models for studying cell migration, differentiation, survival, and growth.
Organoids are miniaturized, simplified versions of organs grown in vitro in three dimensions. Derived from tissue cells, embryonic stem cells, or induced pluripotent stem cells, organoids can self-organize due to their self-renewal and differentiation abilities. Over the past decade, organoid research has advanced significantly, leading to the development of cerebral, gut, liver, thymus, lung, kidney, and retinal organoids.
Introducing Scaffold-free 3D Cell Culture Technique
As a trusted partner in cell research, we offer the CERO 3D Incubator & Bioreactor — a revolutionary solution for easy and reproducible 3D cell culture. This advanced system creates an optimal cell culture environment by precisely monitoring and controlling temperature, pH, and CO₂ levels, making it ideal for cultivating stem cells, spheroids, organoids, and tissues.
Key Benefits of the CERO 3D Incubator & Bioreactor:
- Enhanced cell viability and maturation
- Gentle cultivation without shear forces
- No embedding substrate needed
- Long-term cultivation up to more than one year with high homogeneity
With easy setup, simple workflows, and hands-on time below 2 mins per day, the CERO 3D significantly reduces both labor and operational costs, making it a highly efficient tool for advanced 3D cell culture.
CERO 3D Incubator and Bioreactor
Experience Fast and Reliable Spheroid Formation
Designed to address the challenges of spheroid culture, CEROplate ultra-low attachment, offers unmatched performance and reliability, setting a new standard in three-dimensional cell culture.
The ultra-low attachment CEROplate, simplifies the
process for growing 3D aggregates. It features clear
wells with U-bottom or flat-bottom to make sample
monitoring simple. The unique well geometry of the
microplate aids in the formation of an unattached,
round-shaped, single spheroid or organoid in the
center of each well. This allows to assay and analyze 3D aggregates in the same plate without transfer.
CEROplate, Ultra Low Attachment - available as 6 and 24 well plates (flat-bottom) and as 96 and 384 well plates (U-bottom).
Cytokines and Growth Factors
Highest grade at lowest price – Authentic human proteins.
Invite our experts and lift your research to the next stage with a demo 3D CERO Incubator and Bioreactor from OLS
Join OLS and make your research reliable with our 3D CERO Incubator and Bioreactor with your lab-services as results.