Biobanking Workflow: Isolation, Counting, Cryopreservation

From separating mononuclear cells to counting, cultivating and cryopreserving them: end-to-end biobanking solutions from trusted partner brands and our own production in Bremen.



Contact Us

PBMC Biobanking

Cell Biobanking

Tissue Biobanking

Biobanking Solutions for Life Science Research

Biobanking has become a cornerstone of modern life science research. Across the globe, hundreds of millions of biological samples - from humans, animals, and plants - are collected, processed, and stored every year. These samples form the foundation for breakthroughs in cell biology, drug discovery, diagnostics, and personalized medicine.

High-quality biobanking is not just about storage. It requires reliable workflows for cell isolation, counting, cultivation, analysis, and preservation to ensure reproducible results and long-term data integrity.

At OLS, we provide end-to-end biobanking solutions by combining trusted partner brands with products manufactured in our facility in Bremen, Germany.

Complete Biobanking Workflow Solutions

We support every stage of your biobanking process:

  • Separation of mononuclear cells from blood
  • Dissociation reagents for cells or tissue
  • 2D & 3D cell culture systems
  • Cryopreservation media & vials
  • Cell counting & analytical technologies
  • Imaging & flow cytometry solutions

Our portfolio includes:

Biobanking Applications

Our solutions are tailored for:

  • Blood / PBMC biobanking
  • Cell biobanking (cell lines, stem- or hybridoma cells)
  • Tissue biobanking
Biobanking workflow: blood, cells and tissue pass through separation, cultivation and dissociation into sample preparation and storage

PBMC Biobanking Workflow

PBMC biobanking workflow: blood, separation, PBMC, preparation and storage, with counting and analysis throughout

Separation

Peripheral Blood Mononuclear Cells (PBMCs) are efficiently isolated using HiSep™ Lymphocyte Separation Media (LSM1077) and related solutions.

Preparation

We offer optimized cryopreservation media:

  • CryoXL™ with DMSO + Serum
  • CryoXL™ Serum-Free options

Storage

Cryo vials designed for:

  • Liquid nitrogen storage
  • Ultra-low temperature freezers

Counting & Analysis

The CASY Cell Counter & Analyzer ensures

  • Accurate cell counts
  • Viability assessment
  • Volume-based size distribution (lymphocytes vs. monocytes)

More analytical solutions are available usingFlow CytometryandImaging

CASY vivo cell counter beside a laptop showing a size distribution curve
CASYVivo Cell Counter and Analyzer
TIGR tissue grinder and dissociator

Tissue Dissociation


TIGR Tissue Grinder & Dissociator is an enzyme-free approach for generating single cells from tissue.

CASY vivo cell counter with waste and CASYton bottles

Cell Counting & Viability

CASY Cell Counter & Analyzer allows reliable counting of different cell types, as well as fast viability analyses.

CERO 3D bioreactor and incubator

3D Cell Culture Incubation

CERO 3D Incubator & Bioreactor ensures automated cultivation of stem cells, spheroids, organoids and even tissues.

Cell Biobanking

Cell biobanking workflow: cells pass through expansion, dissociation and preparation into storage, with counting and analysis throughout

Cell Biobanking requirements vary depending on the cell type and application. From expansion and dissociation to cryopreservation and analysis, each step must be optimized to ensure cell integrity, viability, and reproducibility.

OLS provides a complete portfolio of solutions to support reliable and scalable cell biobanking workflows.

Cell Expansion

Efficient cell expansion is critical for successful biobanking. While traditional 2D culture systems remain widely used, advanced 3D cell culture technologies offer significant advantages. Our portfolio includes:

  • Classical and specialty cell culture media
  • Supplements and reagents
  • Advanced cell culture systems

3D Cell Culture Advantage

The CERO 3D Incubator & Bioreactor enables:

  • Scalable expansion of pluripotent stem cells and other cell types
  • Higher cell yields
  • Reduced operational costs
  • Maintenance of stem cell pluripotency

Dissociation Reagents

We offer a broad range of dissociation solutions to fit your specific application:

  • Enzymatic and non-enzymatic reagents
  • Gentle cell handling for sensitive cell types
  • Animal component-free options available

Cryopreservation (Preparation)

Different cell types require tailored cryopreservation strategies. The CryoXL™ Cell Freezing Media portfolio includes:

  • Optimized formulations for sensitive, stem, and hybridoma cells
  • DMSO- or glycerin-based media
  • Serum-containing and serum-free options

Storage

Secure and reliable storage is essential for long-term sample integrity.

  • Cryo vials (0.5–5 ml)
  • Suitable for liquid nitrogen and ultra-low temperature storage

Counting & Analysis

Accurate cell analysis is key to ensuring high-quality biobanking results.

The CASY Cell Counter & Analyzer delivers:

  • High precision and reproducibility
  • Flexible analysis for various cell types
  • Reliable quality control before freezing and after thawing
  • Advanced measurement of sensitive cells such as iPSC and ESC
  • Unique volume-based aggregation correction

Additional analytical capabilities are available through Flow cytometryandadvanced imaging solution.

CASY vivo cell counter beside a laptop showing a size distribution curve
CASYVivo Cell Counter and Analyzer

Tissue Biobanking

Tissue biobanking workflow: tissue passes through dissociation, organoid formation, cultivation and preparation into storage

Tissue- and Cell Dissociation Reagents, 3D Organoid formation and cultivation, Cryo-Media and Vials as well as counting and analytical – solutions that support you throughout the process.

Dissociation Reagents:

A broad range of Tissue Dissociation Reagents – the right one for most applications See Dissociation Reagents

Organoid Formation and Cultivation:

CERO 3D Incubator & Bioreactor is a novel solution, allowing even long-term expansion of spheroids and organoids in 3D suspension without shear forces and in most cases without requirement for a supporting matrix.

CERO 3D bioreactor and incubator
CERO 3D bioreactor and incubator, front view
Researcher holding a CEROtube in front of the CERO 3D bioreactor

Preparation:

Different type of cells have different requirements for cryo media. The CryoXL™ Cell Freezing Medium product line provides special solutions for sensitive-, stem- or hybridoma cells containing DMSO or Glycerin. If required Serum free media are available too. For More Details seeCryoXL™ – Cryopreservation.

Storage:

Cryo Vials are available in volumes ranging from 0.5 to 5ml designed for storage in liquid nitrogen and ultra-low freezers. For details see Cryo Vials.

Counting & Analysis:

Especially when standardization is key to success, having reliable information about cell concentration and status is absolutely essential and can be achieved using the CASY Cell Counter and Analyzer . Representative number of cells measured and analyzed in seconds result in low standard deviations.

Additional analytical capabilities are available through Flow cytometryandadvanced imaging solution.

Ihr dynamisches Snippet wird hier angezeigt ... Diese Meldung wird angezeigt, weil Sie weder einen Filter noch eine Vorlage zur Verwendung bereitgestellt haben

Register here for recent updates, events and webinar trainings for CERO 3D Incubator and Bioreactor from OLS

Join OLS and make your research reliable with our CERO 3D Incubator and Bioreactor with your lab-services as results.

Webinar Registration