Live-Cell Imaging and Real-Time Impedance Monitoring


xCELLigence RTCA eSight - Real-Time Insights into Cell Health, Behavior, and Function.


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Key Facts

Applications

FAQ

Literature

Your Challenges using Traditional Methods​​

Only end-point data available.

Invasive & destructive techniques. 

Low sensitivity and delayed detection. 

Poor reproducibility and high variability. 

Lack of multiparametric insights.

Time-consuming and labor-intensive. 

xCELLigence RTCA eSight delivers the solution​

  • Continuous real-time monitoring
  • Label-free impedance technology combined with live-cell imaging
  • Increased reproducibility and efficiency
  • Higher sensitivity for early detection
  • Intuitive data aquisition RTCA software
  • Built-in 21 CFR Part11 compliance, and GMP support

​xCELLigence RTCA eSight: Simplify your workflow and gain physiologically relevant real-time data with ease.

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Advanced Live-Cell Analysis with xCELLigence eSight

Impedance Monitoring & Live-Cell Imaging at the Same Time 

Integrated Impedance & Imaging

Combine label-free real-time impedance analysis with high-resolution live-cell imaging in red, green, and blue, utilizing 5x, 10x, and 20x objectives for enhanced confidence in your results.

Whole-Well Imaging

Expand your view with whole-well imaging, enabling thorough QC, precise cell growth monitoring, and accurate quantification.​ 

Multiplexed Assays

The power of multiplexing offers information “richness”. The eSight allows the tracking of cell health and behavior simultaneously, and of the same cell populations, from the very different vantage points of real-time cellular impedance and live cell imaging.

Very Fast

Read a 96-well plate in 15 seconds with the xCELLigence impedance technology, and image an entire 96-well plate in only 6 minutes.

Flexible Imaging 

Utilize brightfield and three fluorescence channels across various microplate formats (6- to 384-well) and vessel types (35- or 60-mm dishes), with user-defined imaging schedules for maximum flexibility.

Physiologically Relevant  Data in 2D & 3D Cultures​

Easily monitor cell health, behavior, and function with a simple workflow in primary or native cells alone or in co-culture, providing unprecedented insight into cellular mechanisms and functionality.

Applications


Immune Cell Killing

 Cytotoxicity Monitoring

Apoptosis & Proliferation

Cell Migration and Invasion

Viral Cytopathic Effects

Phagocytosis

Cell Adhesion 

 3D Spheroids

​Cell Barrier Function  

Stem Cells   

Cell Signaling Monitoring 

3D Spheroid Invasion

Study invasive potential of tumor spheroids over time as you measure and quantify metastatic potential by label or label-free analysis. Investigate response to treatments and the inhibitory effect on invasion and progression.

Dilution cloning

Easily track cell monoclonal stable cell lines over time using a limited dilution cloning method on eSight. Automatically screen clonal cell lines with minimal effort and high efficiency.

Wound Healing Migration and Invasion

Assess the effects of treatments on cell migration profiles and investigate the rate of wound closure. Explore metastatic potential and how various conditions affect or delay healing. Metrics such as wound width, confluence, cell index, object count, and density are easily measurable with automated software.

Cell-by-cell analysis

Continuously monitor and quantify single cell segmentation by label-free analysis. Gather cell count and nuclear fluorescent recognition count as a function over time. Measure cell subpopulations over time and gain insights into cellular heterogeneity.

Immunotherapy 

Now easily calculate and convert to % cytolysis using imaging parameters to expand your Immunotherapy and Immune Cell Killing Analysis and measure potency with ease.

Literature

Application Notes 

Real-Time Potency Assay for CAR T Cell Killing of Adherent Cancer Cells

Bispecific T Cell Engager (BiTE) Mediated Cancer Cell Lysis

A Novel Real-Time Co-Culture Assay for Immune Cell Invasion and Cytotoxicity

  Real-Time Antibody Internalization Assay

Visualization and Quantitative Analysis of Macrophage Phagocytosis

Monitoring Cell Proliferation in Real Time

Monitoring Drug-Mediated 3D Tumor Spheroid Shrinkage in Real Time 

Real-Time Monitoring and Quantifying 3D Tumor Spheroid Invasion

Real-Time, Multiday Cell Stress Assay

Technical Notes

Limiting Dilution Method Using the xCELLigence RTCA eSight System

Handbooks

Infectious Disease Applications 

Evaluating Immune Cell Therapy Potency

Cancer Immunotherapy

Vaccine and Virology Applications

FAQ

The system measures electrical impedance to track cell adhesion, proliferation, morphology, and viability in real-time, while simultaneous live-cell imaging captures detailed visual data of cellular events.

✔ Real-time, continuous monitoring – No need for endpoint assays.

✔ Label-free impedance technology – No dyes or stains required.

✔ Simultaneous imaging & functional analysis – Brightfield & fluorescence imaging with quantitative impedance data.

✔ High sensitivity – Detects early changes in cell viability, adhesion, and apoptosis.

✔ Improved reproducibility & efficiency – Automated, hands-free data collection.

The system supports a wide range of cell-based assays, including:

  • Cytotoxicity & drug screening
  • Cell proliferation & viability
  • Apoptosis & cell death analysis
  • Immune cell-mediated killing assays
  • Cell adhesion & morphology studies


Unlike endpoint assays (e.g., MTT, Trypan Blue) that require staining or cell lysis, impedance monitoring is non-invasive, allowing for continuous, real-time tracking of cell health and behavior.




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