Live-Cell Imaging and Real-Time Impedance Monitoring

xCELLigence RTCA eSight - Real-Time Insights into Cell Health, Behavior, and Function.

Your Challenges using Traditional Methods

Only end-point data available.

Invasive & destructive techniques.

Low sensitivity and delayed detection.

Poor reproducibility and high variability.

Lack of multiparametric insights.

Time-consuming and labor-intensive.

xCELLigence RTCA eSight delivers the solution

Continuous real-time monitoring
Label-free impedance technology combined with live-cell imaging
Increased reproducibility and efficiency
Higher sensitivity for early detection
Intuitive data acquisition RTCA software
Built-in 21 CFR Part 11 compliance, and GMP support

xCELLigence RTCA eSight: Simplify your workflow and gain physiologically relevant real-time data with ease.

Download brochure: xCELLigence RTCA eSight

Advanced Live-Cell Analysis with xCELLigence eSight

Impedance Monitoring & Live-Cell Imaging at the Same Time

Integrated Impedance & Imaging

Combine label-free real-time impedance analysis with high-resolution live-cell imaging in red, green, and blue, utilizing 5x, 10x, and 20x objectives for enhanced confidence in your results.

Whole-Well Imaging

Expand your view with whole-well imaging, enabling thorough QC, precise cell growth monitoring, and accurate quantification.

Multiplexed Assays

The power of multiplexing offers information “richness”. The eSight allows the tracking of cell health and behavior simultaneously, and of the same cell populations, from the very different vantage points of real-time cellular impedance and live cell imaging.

Very Fast

Read a 96-well plate in 15 seconds with the xCELLigence impedance technology, and image an entire 96-well plate in only 6 minutes.

Flexible Imaging

Utilize brightfield and three fluorescence channels across various microplate formats (6- to 384-well) and vessel types (35- or 60-mm dishes), with user-defined imaging schedules for maximum flexibility.

Physiologically Relevant Data in 2D & 3D Cultures

Easily monitor cell health, behavior, and function with a simple workflow in primary or native cells alone or in co-culture, providing unprecedented insight into cellular mechanisms and functionality.

Applications

Immune Cell Killing

Cytotoxicity Monitoring

Apoptosis & Proliferation

Cell Migration and Invasion

Viral Cytopathic Effects

Phagocytosis

Cell Adhesion

3D Spheroids

Cell Barrier Function

Stem Cells

Cell Signaling Monitoring

3D Spheroid Invasion

Study invasive potential of tumor spheroids over time as you measure and quantify metastatic potential by label or label-free analysis. Investigate response to treatments and the inhibitory effect on invasion and progression.

Spheroid invasion in brightfield beside a graph of invading cell area over 120 hours at ten cytochalasin D concentrations

Dilution cloning

Easily track cell monoclonal stable cell lines over time using a limited dilution cloning method on eSight. Automatically screen clonal cell lines with minimal effort and high efficiency.

Colony growth in a dish beside a graph of red object count over 250 hours

Wound Healing Migration and Invasion

Assess the effects of treatments on cell migration profiles and investigate the rate of wound closure. Explore metastatic potential and how various conditions affect or delay healing. Metrics such as wound width, confluence, cell index, object count, and density are easily measurable with automated software.

Wound closure in three brightfield images beside a graph of wound width over time at ten cytochalasin D concentrations

Cell-by-cell analysis

Continuously monitor and quantify single cell segmentation by label-free analysis. Gather cell count and nuclear fluorescent recognition count as a function over time. Measure cell subpopulations over time and gain insights into cellular heterogeneity.

Immunotherapy

Now easily calculate and convert to % cytolysis using imaging parameters to expand your Immunotherapy and Immune Cell Killing Analysis and measure potency with ease.

Target cell nuclei in red and T cells in blue after one and twenty hours, beside a graph of cytolysis over 90 hours at nine CAR-T cell numbers

Consumables

xCELLigence E-Plate 96

RTCA Microplates, Kits & Accessories

Literature

Application Notes

Real-Time Potency Assay for CAR T Cell Killing of Adherent Cancer Cells

Bispecific T Cell Engager (BiTE) Mediated Cancer Cell Lysis

A Novel Real-Time Co-Culture Assay for Immune Cell Invasion and Cytotoxicity

Real-Time Antibody Internalization Assay

Visualization and Quantitative Analysis of Macrophage Phagocytosis

Monitoring Cell Proliferation in Real Time

Monitoring Drug-Mediated 3D Tumor Spheroid Shrinkage in Real Time

Real-Time Monitoring and Quantifying 3D Tumor Spheroid Invasion

Real-Time, Multiday Cell Stress Assay

Technical Notes

Limiting Dilution Method Using the xCELLigence RTCA eSight System

Handbooks

Infectious Disease Applications

Evaluating Immune Cell Therapy Potency

Cancer Immunotherapy

Vaccine and Virology Applications

FAQ

The system measures electrical impedance to track cell adhesion, proliferation, morphology, and viability in real-time, while simultaneous live-cell imagingcaptures detailed visual data of cellular events.

✔ Real-time, continuous monitoring – No need for endpoint assays.

✔ Label-free impedance technology – No dyes or stains required.

✔ Simultaneous imaging & functional analysis – Brightfield & fluorescence imaging with quantitative impedance data.

✔ High sensitivity – Detects early changes in cell viability, adhesion, and apoptosis.

✔ Improved reproducibility & efficiency – Automated, hands-free data collection.

The system supports a wide range of cell-based assays, including:

Cytotoxicity & drug screening
Cell proliferation & viability
Apoptosis & cell death analysis
Immune cell-mediated killing assays
Cell adhesion & morphology studies

Unlike endpoint assays (e.g., MTT, Trypan Blue) that require staining or cell lysis, impedance monitoring is non-invasive, allowing for continuous, real-time tracking of cell health and behavior.

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