Advanced 3D Cell Culture - Enhanced Cell Viability, Easy Handling, Budget-friendly

CERO 3D incubator and Bioreactor: optimized cultivation of stem cells, spheroids, organoids and tissue biopsies

Trusted by hundreds of labs worldwide

Save time, enhance quality.

Prof. Dr. L. Sevenich and Anna Wolfram | University Hospital Tübingen

Scalable cultivation of iPSCs.

Dr. J. Neubauer | Managing Director at Fraunhofer Project Center for Stem Cell Process Technology

Advanced liver tissue research.

Prof. Dr. A. Nüssler and Prof. Dr. S. Ehnert | Siegfried Weller Institute Tübingen

Your Challenges in 3D Cell Culture

Differentiation of pluripotent stem cells results in heterogeneous cell populations.

Long-term culture of organoids leads to loss of structure and metabolic activity.

Time consuming manual maintenance like media changes.

High costs for consumables and substrates.

Solved by CERO 3D Incubator and Bioreactor

Increased cell vitality and homogeniety of cell populations

Cost-efficient cultivation with less media and no embedding substrates needed

Ideal for short- and long-term culture

Special culture vessels ensure minimal hands-on time for media change

CERO 3D - Key Facts

Enhanced Cell Viability and Vitality

CERO 3D’s low-shear bi-directional rotation minimizes cell stress and damage, promoting long-term culture, survival, and differentiation without compromising cell health.

Flexible & Scalable

CERO 3D supports a wide range of cell types—including stem cells, tumor cells, and even tissue biopsies—offering scalable 3D culture in four individually controlled tubes, all in a compact benchtop footprint.

Automated Environmental Control

Precise control of CO₂, temperature, and pH ensures optimal conditions for growing and differentiating organoids, tissue biopsies, and other 3D structures.

Budget-friendly & Easy handling

Intuitive operation with minimal hands-on time—quick 4-minute media changes, reduced media consumption, and no need for embedding substrates.

Extended Culturing for Long-Term Studies

Designed for long-term studies, CERO 3D supports cultures from days to months—ideal for regenerative medicine, cancer research, and viral studies.

Advanced 3D Cell Culture

Achieve more realistic cell behavior and interactions with 3D culture—enhancing accuracy in cancer research, tissue modeling, and regenerative medicine.

Trusted by Experts

"The generation of gastric organoids is a crucial step in the study of Helicobacter pylori infection and gastric carcinogenesis. The CERO 3D Incubator & Bioreactor allows the generation in much more efficient, reproducible, physiological and cost efficient way compared to any other approach."

Dr. Raquel Mejias-Luque • Technical University of Munich

"With the CERO 3D we are implementing and developing tools for patient-specific tumor therapy. Furthermore, the cancer cells isolated from fresh tumour biopsies make treatment optimization possible before the therapy is initiated. Here, CERO 3D incubator & bioreactor enable us to develop personalized medicine in a 3D cell culture model."

Prof. Dr. Rückert • University Hospital Mannheim

“The overarching aim of all our studies is the identification and development of clinically relevant therapeutic agents for the treatment of human heart disease. To that end we utilize human self-organised cardiac organoids for high throughput drug screening and in vivo genetic models of human congenital disease, as our experimental platform, in combination with adeno-associated virus (AAV) and anti-sense RNAs as therapeutic agents. The CERO 3D has considerably simplified our cardiac organoid generation workflow and enabled us to scale-up organoid production significantly. Organoids generated with CERO 3D reveal improved cellular organisation, and display uniformity and consistency within and across production batches.”

Prof. Dr. Jaya Krishnan • Institute of Cardiovascular Regeneration, Goethe-University Frankfurt • Co-Founder of Genome Biologics

"...Moreover, this 3D cultivation system substantially improves viability, maturation and contractility, therefore provides a reliable tool for cardiovascular therapies."

Yvonne Eibach • Max Planck Institute for Lung and Heart Research

"Cultivating hepatocyte shperoids in CERO 3D considerably improves expansion, differentiation, maturation and hepatic virus infection compared to monolayer culture. Our research takes advantage from healthy cells even from long-term cultures in CERO 3D. Moreover, we are now able to perform 3D long-term culture of human tissue specimen in CERO 3D - a paradigm shift."

Prof. Dr. Heikenwälder • M3 Research Center • University Hospital Tübingen

Applications

"With CERO 3D, we increased the size of our organoids by 50% while cutting manual labor in half. Our research is now more precise and efficient." - Dr. Anna Miller, Head of Cancer Research, Vienna.