Image-Based Single Cell Isolation & Monoclonal Colony Growth
CellRaft
AIR® System - Automatically image, track and analyze cells or colonies
of interest for downstream endpoint analysis or clonal expansion.
Challenges in Generation of Single Cells and Monoclonal Colonies
Poor survival rates due to high shear stress.
No control over monoclonality.
High manual workload.
High demand for consumables.
Solved by CellRaft AIR®
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Gentle cell handling ensures high viability.
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Non-invasive Monitoring.
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Automated Cell/Colony Isolation.
- Reduced need of media and plastic ware.
How CellRaft AIR® Isolates Single Cells Without Fluidics
10 - 50x More Monoclonal Colonies in Weeks, not Months
Grow single cells in a flask-live environment, ensuring the viability and vitality of single cells while they develop into clones.
Identify and Monitor
Identify
cells of interest using an integrated three-channel fluorescence plus
brightfield microscope with user-defined parameters.
Automated Isolation
Automatically isolate CellRafts containing cells or colonies of interest without the use of fluidics.
Image-Based Proof of Monoclonality
Create
a complete image-based record of all clones to support regulatory submissions.
Technology and Workflow
CellRaft technology revolutionizes the development of monoclonal colonies by eliminating traditional methods like trypsinization and manual cell dispensing.
It uses 3 key components:
CellRaft Array
A specialized tissue culture dish containing thousands of microscale cell culture growth surfaces called CellRafts that provide a stable, supportive environment for single-cell growth.
CellRaft
Cytometry™ Software
Advanced software designed for precise analysis and monitoring of cell behavior.
CellRaft AIR Platform
An integrated system that combines rapid imaging, software-guided identification, and automated isolation for high-efficiency monoclonal colony development.
CellRaft AIR® Technology
CellRaft AIR® System
Applications
Monoclonal lines with image-based proof.
Find edited clones before expansion.
iPSC colonies without enzymatic passaging.
Organoids from single cells, tracked.
Single cells retrieved for sequencing.
Recover antibody-secreting cells, still viable.
FAQs
The CellRaft AIR System is an advanced platform for single-cell isolation and analysis. It allows researchers to track, identify, and retrieve individual cells while maintaining their viability and native expression profiles.
It is ideal for applications such as:
- Cell line development
- Stem cell culture
- Organoid and spheroid research
- Single cell genomics
- CRISPR Gene editing
The system uses single-cell-resolution imaging to capture cell morphology and growth dynamics, allowing identification and tracking of cells based on user-defined phenotypic criteria.
The system isolates cells of interest without using any fluidics and without enzymatic treatments (like trypsin), preserving their natural state and minimizing stress.
It combines imaging, software-driven selection, and automated cell retrieval into one seamless workflow, reducing manual handling and improving cell recovery rates.
Get in touch with one of our specialists and speed the process of generating single cells with CellRaft AIR®
Downloads
Flyer and application notes for the CellRaft AIR® System.