Despite advancements, challenges remain in cell line development, especially in generating single-cell clones. Traditional isolation methods like limiting dilution and single-cell dispensing, while cost-effective, present several issues:

• Low clone viability
• Uncertainty of monoclonality
• High demand for lab supplies and equipment
• Extensive hands-on time
• Limited throughput

These obstacles hinder efficiency and scalability in cell engineering.

 The CellRaft AIR® System is the ideal platform for accelerating cell line generation with its unique capabilities:

• Reduced Development Timeline
• Seamless Workflow Integration
• Centralized Data for Archiving & Application Submissions

Utilizing the CellRaft® Array, a specialized culture dish, it enables screening of thousands of cells while ensuring single-cell segregation.

• High-Throughput Screening: Efficiently screens thousands of cells on a single dish for rapid results.
• Monoclonality Proof: Provides image-based proof of monoclonality, ensuring single clones for experiments and downstream applications. Over 100 cell lines tested.
• Clonal Isolation: Isolates entire clones, improving viability and streamlining downstream processes.
• Flask-Like Culture Conditions: Cells share media and growth factors, mimicking their natural environment for optimal growth.
• Gentle Clonal Isolation: The absence of fluidics or pressure minimizes cellular stress during the transition to collection plates.

Flask-like Culture Conditions + Single-cell Separation + Image-based, Software-guided Selection = Automated Retrieval of High Vitality Cells, Colonies, or Organoids

• Faster Isolation: CellRaft technology accelerates single-cell isolation workflows, reducing consumables and media use by over 80%.
• Time-Course Imaging & Trackability: Continuous imaging captures full growth records for easy analysis and permanent archiving.

• Higher Clonal Outgrowth Efficiency: Supports healthy clone development by maintaining a natural growth environment without physical separation of cells.

Clonal outgrowth efficiency of CHO-K1 cells in n=3 96-well plates using Limiting Dilution, CellRaft AIR 100S and 200S formats.

Unlike traditional platforms that require multiple units, the CellRaft AIR® System integrates cloning, imaging, expansion, and screening into one seamless solution, reducing complexity, cost, time, and error rates.

CLD Workflow