Overcoming Bottlenecks in Cell Line Development

Minimize the Guesswork. Maximize the Output.

Challenges in Cell Line Development

Despite advancements, challenges remain in cell line development, especially in generating single-cell clones. Traditional single-cell isolation methods like limiting dilution and single-cell dispensing, though cost-effective, present several challenges:

Lack of viable clones

Lack of proof of monoclonality

High demand for lab supplies and equipment

Extensive hands-on time

Limited throughput

At Colossal Biosciences, we only work with non-model organisms and primary cells and cell lines that haven[t been studied before. These cells often fail to grow out after monoclonal isolation using flow sorting. In the few months since adopting the CellRaft, we have isolated thousands of validated monoclonals from elephants, canids and deer with consistent outgrowth efficiencies."

Dr. Sven Bocklandt • Species Director at Colossal where he is leading a team of genome engineers and cell biologists focused on bringing back extinct animal species.

The Solution: CellRaft Technology

The CellRaft AIR® System stands out as the preferred platform for accelerating cell line generation

Reduced development timelines
Seamless workflow integration
Centralized data for archiving and application submissions

Utilizing the CellRaft® Array, a specialized culture dish, enables screening of thousands of cells while ensuring single-cell segregation.

Key Advantages

High-Throughput Screening

Screen thousands of cells simultaneously in a single culture dish, allowing for more efficient and rapid screening.

Monoclonality Proof

Track and trace image-based proof of monoclonality with brightfield and fluorescence.

Flask-Like Culture Conditions

Cells share media and growth factors, mimicking their natural environment for optimal growth.

Non-destructive Isolation

Automatically transfer single cells or entire clones of interest into 96 well-plates without any fluidics to minimize cellular stress and maximize viability.

flask culture conditions

Flask-like Culture Conditions + Single-cell Separation + Image-based, Software-guided Selection = Automated Retrieval of High Vitality Cells, Colonies, or Organoids

Proven Benefits

Faster Isolation: CellRaft technology accelerates single-cell isolation workflows, reducing consumables and media use by over 80%.

Time-Course Imaging & Trackability: Continuous imaging captures full growth records for easy analysis and permanent archiving.

Higher Clonal Outgrowth Efficiency: Supports healthy clone development by maintaining a natural growth environment without physical separation of cells.

Left: Clonal outgrowth efficiency of CHO-K1 cells in n=3 96-well plates using Limiting Dilution, CellRaft AIR 100S and 200S formats.

Streamlined Workflow

Unlike traditional platforms that require multiple units, the CellRaft AIR® System integrates cloning, imaging, expansion, and screening into one seamless solution, reducing complexity, cost, time, and error rates.

Cost and Time Efficiency

Studies show CellRaft Technology significantly improves outgrowth efficiency, saving time and costs compared to limiting dilution methods.

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