CellRaft Technology enhances iPSC
viability and monoclonal colony formation by providing flask-like culture
conditions while maintaining single-cell separation. A single CellRaft Array
can screen thousands of iPSCs, accelerating cell line generation and therapeutic
discovery while simplifying iPSC maintenance workflows.
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25X higher iPSC clonal generation than limiting dilution
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Track & trace iPSCs from single cell to clone
- Expand, characterize, edit, and reprogram with ease
- Confirm pluripotency on-array before clonal expansion
- Reduce time, consumables, and labor for iPSC development
- Enable 2D & 3D applications, including differentiation and reprogramming
- Fully automate isolation of viable, validated iPSC cell lines
Increase Monoclonal Colony Generation and Outgrowth
Screen 60,000+ iPSCs per array — 500X more cells than a traditional 96-well plate with limiting dilution.
Figure 1. Rather than isolating a single cell, the isolation is done once the single cell has grown into a colony. The CellRafts in the experiment above were isolated at different growth stages and the success rate of outgrowth increases significantly when cells are isolated at the colony stage.
Application Note:
An automated solution to ipsc reprogramming
Track and Trace from Single Cell to Clone
Follow the maturation of a clone as it grows from a single cell.
Figure 2. Track and trace of iPSC clone formation
on the CellRaft Air. Four different iPSC cell lines were seeded on CellRaft
arrays on one of three coatings (iMatrix-511, h-ESC Matrigel, or Laminin). The
arrays were serially scanned starting 4 hours post-cell seeding and every 24
hours after to monitor clone formation.
Enable All iPSC Worfklows Using CellRaft Technology
1. Expand the clonal population on the array.
2. Characterize for phenotyping or pluripotency.
3. Differentiate into 3D tissues or organoids.
4. Reprogram differentiated fibroblasts into pluripotent stem cells.
Reduce Time, Costs, and Lab Resources
iPSC workflows are often expensive and labor-intensive. CellRaft Technology streamlines the process, significantly cutting costs and reagent use without compromising viability.
- Generate 200+ iPSC clones per array vs. 10 per 96-well plate (limiting dilution)
- Require 1000X less iPSC coating and 2000X less media per cell screened
Grow & Isolate iPSC-Derived Organoids
CellRaft
Technology enables the growth and long-term maintenance of hundreds of
individual organoids. With serial imaging, you can track development and
phenotypically characterize organoids to select the most relevant models.
Figure 3: iPSC-Derived Organoid Formation. CellRaft Cytometry™ software tracks
iPSC seeding to organoid development, allowing users to define desired
characteristics. The example shows 100–300µm organoids at day 10, with hundreds
generated per array.
Figure 4: Cerebral Organoid Differentiation. A single green organoid is identified on day 1, expands by day 10, and is then isolated into a collection plate, where it continues to grow.