CellRaft Technology enhances iPSC viability and monoclonal colony formation by providing flask-like culture conditions while maintaining single-cell separation. A single CellRaft Array can screen thousands of iPSCs, accelerating cell line generation and therapeutic discovery while simplifying iPSC maintenance workflows.

Key Benefits:

• 25X higher iPSC clonal generation than limiting dilution
• Track & trace iPSCs from single cell to clone
• Expand, characterize, edit, and reprogram with ease
• Confirm pluripotency on-array before clonal expansion
• Reduce time, consumables, and labor for iPSC development
• Enable 2D & 3D applications, including differentiation and reprogramming
• Fully automate isolation of viable, validated iPSC cell lines

Increase in iPSC Monoclonal Colony Generation and Outgrowth

Screen 60,000+ iPSCs per array — 500X more cells than a traditional 96-well plate with limiting dilution.

Figure 1. Rather than isolating a single cell, the isolation is done once the single cell has grown into a colony. The CellRafts in the experiment above were isolated at different growth stages and the success rate of outgrowth increases significantly when cells are isolated at the colony stage.

Track and Trace from Single Cell to Clone

Follow the maturation of a clone as it grows from a single cell.

Figure 2. Track and trace of iPSC clone formation on the CellRaft Air. Four different iPSC cell lines were seeded on CellRaft arrays on one of three coatings (iMatrix-511, h-ESC Matrigel, or Laminin). The arrays were serially scanned starting 4 hours post-cell seeding and every 24 hours after to monitor clone formation.

• Expand the clonal population on the array
• Characterize for phenotyping or pluripotency
• Differentiate into 3D tissues or organoids
• Reprogram differentiated fibroblasts into pluripotent stem cells

iPSC workflows are often expensive and labor-intensive. CellRaft Technology streamlines the process, significantly cutting costs and reagent use without compromising viability.

• Generate 200+ iPSC clones per array vs. 10 per 96-well plate (limiting dilution)
• Require 1000X less iPSC coating and 2000X less media per cell screened

CellRaft Technology enables the growth and long-term maintenance of hundreds of individual organoids. With serial imaging, you can track development and phenotypically characterize organoids to select the most relevant models.

Figure 3: iPSC-Derived Organoid Formation. CellRaft Cytometry™ software tracks iPSC seeding to organoid development, allowing users to define desired characteristics. The example shows 100–300µm organoids at day 10, with hundreds generated per array.

Figure 4: Cerebral Organoid Differentiation. A single green organoid is identified on day 1, expands by day 10, and is then isolated into a collection plate, where it continues to grow.