30-Color Immunophenotyping with NovoCyte Penteon
Abstract
Use of flow cytometry has become routine across scientific disciplines, encompassing both the basic and clinical research spaces. It has proven to be a powerful tool to analyze different subpopulations of immune cells and gather a comprehensive overview of the immune system. Due to great advancements within the field, newer flow cytometers can support multiple colors, enabling identification of a variety of cells simultaneously. In this application note, a 30-color immunophenotyping panel was designed for the five-laser Agilent NovoCyte Penteon flow cytometer to see the distribution of immune cells in the lung, spleen, and mediastinal lymph nodes of mice infected with influenza.
Conclusion
The Agilent NovoCyte Penteon flow cytometer enables
researchers to study multiple important parameters in one
acquisition—a great asset in immunophenotyping. With
five lasers and 30 detectors, it is possible to extract more
information from a single tube, saving precious biological
material. Absolute count provides even more information
about the sample. In this application note, the same gating
strategy was applied on three lymphoid organs, permitting
comparison of T cell distribution and allowing insight into
the immune system during influenza infection. The high
detection capabilities of the NovoCyte Penteon also allowed
identification of other immune cell subsets in the lung, spleen,
and lymph nodes in the same experiment
Enhance your immune cell analysis with NovoCyte Penteon. With five lasers
and 30 detectors, this advanced flow cytometer enables multi-parameter
immunophenotyping, providing precise, high-throughput immune profiling while preserving valuable samples.
Revolutionize your research with 30-color immunophenotyping. Request a free
demo today!
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