Cell Analysis

 Cell Analysis

A 30-color Immunophenotyping Panel of mice infected with Influenza using the Agilent Novocyte Penteon Flow Cytometer 

A 30-color Immunophenotyping Panel of mice infected with Influenza using the Agilent Novocyte Penteon Flow Cytometer 


Abstract

Use of flow cytometry has become routine across scientific disciplines, encompassing both the basic and clinical research spaces. It has proven to be a powerful tool to analyze different subpopulations of immune cells and gather a comprehensive overview of the immune system. Due to great advancements within the field, newer flow cytometers can support multiple colors, enabling identification of a variety of cells simultaneously. In this application note, a 30-color immunophenotyping panel was designed for the five-laser Agilent NovoCyte Penteon flow cytometer to see the distribution of immune cells in the lung, spleen, and mediastinal lymph nodes of mice infected with influenza.



Conclusion 


The Agilent NovoCyte Penteon flow cytometer enables researchers to study multiple important parameters in one acquisition—a great asset in immunophenotyping. With five lasers and 30 detectors, it is possible to extract more information from a single tube, saving precious biological material. Absolute count provides even more information about the sample. In this application note, the same gating strategy was applied on three lymphoid organs, permitting comparison of T cell distribution and allowing insight into the immune system during influenza infection. The high detection capabilities of the NovoCyte Penteon also allowed identification of other immune cell subsets in the lung, spleen, and lymph nodes in the same experiment

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