Advanced Technology in Living Single Cells
Lab-in-a-cell SPAchip® - A revolutionary solution for monitoring cellular pathways in real-time!
Intracellular silicon microchips are designed to be easily internalized in living cells without inducing physiological changes or affecting cell viability
Dynamic film about cell sensing
SPAchip® technology utilizes intracellular silicon microchips to monitor cellular pathways in real time. These chips can be easily internalized in living cells and printed with multiple fluorescent probes to analyze intracellular processes
Dynamic film about cell sensing - SPAchip Technology.
Enhance Your Research on Cellular Pathways
The SPAchip® is the first intracellular device that can be multiplexed with probes emitting in the same wavelength. Surface functionalization techniques enable SPAchip® to be printed with nucleic acids, proteins, carbohydrates, lipids, or synthetic chemicals for tracking cells.
Thanks to a cutting-edge miniaturization process, the SPAchips offer a highly precise and accurate way to analyze intracellular processes with multiple fluorescent probes. By simply changing the SPAchip® fluorescence, you can gain valuable insights into cellular activity. With the SPAchip® kit, you can save time and costs by using it with high-content screening platforms and imaging analyzers. It offers a user-friendly, yet powerful solution for monitoring cellular pathways that will help you take your research to the next level.
The SPAchip® Difference: A Leap in Live Cell Analysis
Multiplex Capability
SPAchip® is the first intracellular device capable of being multiplexed with probes emitting in the same wavelength, revolutionizing how we study cellular metabolism.
Surface Functionalization
Enables the printing of SPAchips with a wide array of biomolecules, facilitating comprehensive tracking of cellular activities and metabolism.
Non-Invasive
SPAchips are non-toxic, ensuring long-term intracellular measurements can be taken without harming the cell.
Enhanced Sensitivity
By concentrating the fluorescent probe on the chip’s surface rather than in solution, SPAchip® technology offers unparalleled sensitivity in detecting cellular changes.
Eco-Friendly
Utilizes fewer plastics and consumables, aligning with sustainable lab practices.
Versatile Applications
SPAchip´s workflow is designed to complement existing lab infrastructures, integrating seamlessly with a variety of platforms for a wide range of applications.
SPAchip® works in combination with the current laboratory equipments
Explore our comprehensive workflow for analyzing cell-based imaging assays using high-content imaging and cellular analysis software. Our user-friendly assay kits seamlessly integrate with modern cell imaging instruments, ensuring accurate results and enhanced workflow efficiency for life science research, drug discovery, and bioassays.
Accelerating Drug Discovery with SPAchip® Technology
SPAchip® offers real-time monitoring of single cells, revealing new targets and therapeutic opportunities, while enhancing efficiency and safety in drug development for better patient outcomes.
Target-Based Innovation
A4cell’s technology accelerates target-based drug discovery by enabling custom compound design and direct target detection, streamlining the drug development process.
Advancing Phenotypic Discovery Leap
A4cell’s technology enables molecule printing and analysis, aiding in the discovery of new targets, mechanisms of action, and drug candidates, driving therapeutic innovation.
Optimizing HCS Platforms
A4cell enhances High Content Screening (HCS), boosting lead identification, drug efficacy assessment, and cellular phenotype analysis.
SPAchip® technology combined with live cell imaging enables innovative assays, reduces phototoxicity, and uses a single fluorescence channel, cutting time and costs by up to 60%. The A4cell Multiplex Detection kit reveals hidden patterns and correlations, advancing drug discovery and personalized medicine.
Applications and Cell Types
ONCOLOGY
- Cell proliferation
- Apoptosis
- Cell migration
- Tumor Microenvironment
- Inmuno oncology
- Ferroptosis
- Inflammation
METABOLISM
- Oxidative
- Mitochondrial function
- Acidification
- Calcium signaling
- Glucose consumption
- Response to hypoxia
- Autophagy
iPS CELL
- Cell culture quality control
- Differentation processesz
CARDIOVASCULAR
- Mitochondrial function
- Acidification
- Calcium signaling
- Glucose consumption
- Response to hypoxia
- Autophagia
CELL THERAPY
- Cell culture quality control
- Viability and proliferation
- Cell functional properties
Timelapse of HEK293 cell line (from Human Embryonic Kidney) spheroid formation with internalization of CytoCHECK SPAchip® pH Single-Detection Kit Green. DRAQ5™ in red stained live nuclei. SPAchip® technology allows pH measurement at the different time points of spheroid formation. HEK293 cells were incubated with CytoCHECK SPAchip® pH Single-Detection to allow spheroid formation at 24 A), 28 B), 48 C), and 52 hours D).
Consumables
CytoCHECK SPAchip® pH green Single-Detection Kit
Product Reference: S-001-PHG
For research use only (RUO)
CytoCHECK SPAchip® pH red Single-Detection Kit
Product Reference: S-001-PHR
For research use only (RUO)
CytoTRACK SPAchip® pH Dual-Detection Kit
Product Reference: D-001-PHRG
For research use only (RUO)
SpheroCHECK SPAchip® pH Green Single Detection Kit
Product Reference: SS-001-PHG
For research use only (RUO)
CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit
Product Reference: M-001-PC
For research use only (RUO)
CytoCHECKSPAchip® OHrad ROS Single-Detection Kit
Product Reference: S-003-ROSG
For research use only (RUO)
CytoCHECK SPAchip® Calcium Single Detection Kit
Product Reference:
S-002-CAG
For research use only (RUO)
Register here for recent updates, events, webinar trainings and new product launches from OLS
Webinar Registration