Advanced Technology in Living Single Cell
Lab-in-a-cell SPAchip®
A revolutionary solution for monitoring cellular pathways in real-time! Intracellular silicon microchips are designed to be easily internalized in living cells without inducing physiological changes or affecting cell viability.
Dynamic film about cell sensing
SPAchip® technology utilizes intracellular silicon microchips to monitor cellular pathways in real time. These chips can be easily internalized in living cells and printed with multiple fluorescent probes to analyze intracellular processes.
CytoCHECK SPAchip® pH green Single-Detection Kit
Product Reference: S-001-PHG
For research use only (RUO)
CytoCHECK SPAchip® pH red Single-Detection Kit
Product Reference: S-001-PHR
For research use only (RUO)
CytoTRACK SPAchip® pH
Dual-Detection Kit
Product Reference: D-001-PHRG
For research use only (RUO)
SpheroCHECK SPAchip® pH Green Single Detection Kit
Product Reference: SS-001-PHG
For research use only (RUO)
SpheroCHECK SPAchip® pH Green Single-Detection Kit
Product Reference: SS-001-PHG
For research use only (RUO)
CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit
Product Reference: M-001-PC
For research use only (RUO)
CytoCHECKSPAchip® OHrad ROS Single-Detection Kit
Product Reference: S-003-ROSG
For research use only (RUO)
SPAchip® technology can enhance your research on cellular pathways
The SPAchip® is the first intracellular device that can be multiplexed with probes emitting in the same wavelength. Surface functionalization techniques enable SPAchip® to be printed with nucleic acids, proteins, carbohydrates, lipids, or synthetic chemicals for tracking cell metabolism.
Thanks to a cutting-edge miniaturization process, the SPAchips offer a highly precise and accurate way to analyze intracellular processes with multiple fluorescent probes. By simply changing the SPAchip® fluorescence, you can gain valuable insights into cellular activity. With the SPAchip® kit, you can save time and costs by using it with high-content screening platforms and imaging analyzers. It offers a user-friendly, yet powerful solution for monitoring cellular pathways that will help you take your research to the next level
Key Features
The SPAchip® Difference: A Leap in Live Cell Analysis
- Multiplex Capability: SPAchip® is the first intracellular device capable of being multiplexed with probes emitting in the same wavelength, revolutionizing how we study cellular metabolism.
- Surface Functionalization: this technology enables the printing of SPAchips with a wide array of biomolecules, facilitating comprehensive tracking of cellular activities and metabolism.
- Non-Invasive: SPAchips are non-toxic, ensuring long-term intracellular measurements can be taken without harming the cell.
- Enhanced Sensitivity: by concentrating the fluorescent probe on the chip’s surface rather than in solution, SPAchip® technology offers unparalleled sensitivity in detecting cellular changes.
- Eco-Friendly: utilizes fewer plastics and consumables, aligning with sustainable lab practices.
- Versatile Application: SPAchip´s workflow is designed to complement existing lab infrastructures, integrating seamlessly with a variety of platforms for a wide range of applications.
- Highest reproducibility and accuracy for anything you want to analyze
- No sample preparation, easy use
- High speed at low cost
- Transparent results and quality control
- Thousands of publications and thousands of installations
- GMP/GLP compliant
- Optional 21CFR Part 11 module
CASY Cell Counter & Analyzer – always the best choice, whatever you want to analyze. From large stem cell aggregates to small yeast or even bacteria. CASY provides unmatched precision and reproducibility.
CASY can do it all – the unique detection mechanism will support you even with challenging applications
SPAchip® works in combination with the current laboratory equipments
Enhancing Workflow Efficiency with SPAchip® Technology
Below, we present a comprehensive workflow for the analysis of cell-based imaging assays utilizing high-content imaging and cellular analysis software. Our user-friendly assay kits cater to the needs of life science research, drug discovery, and bioassays, providing a seamless experience. These kits are meticulously designed to seamlessly integrate with the latest Cell Imaging instruments, ensuring optimal performance and reliable results.
Revolutionizing Drug Discovery
SPAchip® is not just an innovation in live cell imaging; it’s a transformative tool for drug discovery.
By enabling detailed analysis of single-cell responses to drug candidates, SPAchip® technology accelerates the identi cation of new targets and therapeutic
opportunities, promising a new era of personalized medicine and ef cient drug development.
- Target-Based Innovation: A4cell’s technology enhances target-based drug discovery by facilitating the design and printing of custom chemical compounds. This capability allows for the quick and direct detection of specific targets, streamlining the drug development process.
- Phenotypic Discovery Leap: It advances phenotypic drug discovery by enabling the printing and analysis of various molecules. This breakthrough assists in identifying new therapeutic targets, mechanisms of action, and potential drug candidates, marking a significant stride forward.
- Optimizing HCS Platforms: A4cell optimizes High Content Screening (HCS) platforms, improving the e iciency of identifying lead compounds, assessing drug e icacy, and characterizing cellular phenotypes.
The synergy between live cell imaging and SPAchip® technology fosters the creation of innovative assays and experimental protocols, broadening the horizons of biological research. Additionally, this approach minimizes phototoxicity and uses only one uorescence channel, eliminating the need for extra experiments and saving up to 60% on time and costs. The A4cell Multiplex Detection kit unveils new insights, uncovering patterns and correlations previously hidden, enriching our understanding in drug discovery and steering us
towards personalized medicine.
Applications and Cell Types
Because each cell is a Storyteller….
The SPAchip® abilities to monitor living single cells during drug discovery offers a deeper understanding of drug responses. Leads to the identi cation of new targets and therapeutic opportunities, and can improve the ef ciency and safety of the drug development process. It is a powerful tool that has the potential to accelerate drug discovery and improve patient outcomes.
Timelapse of HEK293 cell line (from Human Embryonic Kidney) spheroid formation with internalization of CytoCHECK SPAchip® pH Single-Detection Kit Green. DRAQ5™ in red stained live nuclei. SPAchip® technology allows pH measurement at the different time points of spheroid formation. HEK293 cells were incubated with CytoCHECK SPAchip® pH Single-Detection to allow spheroid formation at 24 A), 28 B), 48 C), and 52 hours D).
A4cell is delighted to present you a disruptive technology able to analyze living single cells for extended periods offering innovative
Cell-based assays with huge advantages:
VERSATILITY / LACK OF TOXICITY / TRACKING ABILITIES
MULTIPLE ANALYSIS POSSIBILITIES
ONE WAVELENGHT FITS ALL!
Connect us to gain from our SPAchip®technology!
ONCOLOGY
- Cell proliferation
- Apoptosis
- Cell migration
- Tumor Microenvironment
- Inmuno oncology
- Ferroptosis
- Inflammation
METABOLISM
- Oxidative
- Mitochondrial function
- Acidification
- Calcium signaling
- Glucose consumption
- Response to hypoxia
- Autophagy
iPS CELL
- Cell culture quality control
- Differentation processesz
CARDIO-VASCULAR
- Mitochondrial function
- Acidification
- Calcium signaling
- Glucose consumption
- Response to hypoxia
- Autophagia
CELL THERAPY
- Cell culture quality control
- Viability and proliferation
- Cell functional properties
Consumables
CytoCHECK SPAchip® OHrad ROS Single-Detection Kit
SpheroCHECK SPAchip® pH Green Single Detection Kit
CytoCHECK SPAchip® pH red Single-Detection Kit
CytoCHECK SPAchip® pH green Single-Detection Kit
CytoTRACK SPAchip® pH
Dual-Detection Kit
SpheroCHECK SPAchip® pH Green Single Detection Kit
CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit
Register here for recent updates, events, webinar trainings and new product launches from OLS
Webinar Registration