Cell and gene therapies have transformed the treatment of hematologic cancers, but translating these approaches to solid tumors remains challenging. Advanced 3D cell models combined with real-time quantitative imaging are now enabling deeper insights into tumor biology and immune-cell-mediated killing.

Why 3D Tumor Spheroids?

In vitro 3D cultures bridge the gap between traditional 2D assays and animal models. Tumor spheroids closely mimic in vivo tissue architecture, capturing key features such as:

• Cell-cell and cell-matrix interactions
• Hypoxia and central necrosis
• Drug penetration and resistance
• Extracellular matrix (ECM) production and remodeling

These physiologically relevant models provide more predictive data for immuno-oncology and drug discovery.

Real-Time, Quantitative 3D Analysis

xCELLigence cell analysis solutions deliver robust workflows for monitoring immune-cell-driven inhibition of 3D tumor growth and invasion. Integrated incubation, imaging, and analysis enable long-term kinetic studies with minimal hands-on time and high reproducibility.

• Live-cell imaging for real-time monitoring of 3D tumor growth and invasion
• Label-free imaging or simple mix-and-read reagents to assess proliferation and cytotoxicity
• Quantitative insights into morphology and cellular dynamics in mono- and co-culture models
• Simple, reproducible viability and toxicity readouts for deeper biological insight
• Physiologically relevant modeling of tumor heterogeneity and the tumor microenvironment