Leukocyte Subset Detection

Comprehensive 18-Color Pan-Leukocyte Flow Cytometry Analysis for Immune Surveillance

Understanding immune status requires precise monitoring of multiple leukocyte subsets. Using the Agilent NovoCyte Quanteon Flow Cytometer, researchers developed an 18-color immunophenotyping panel to simultaneously identify and quantify a wide range of cell types in human PBMCs — including monocytes, B cells, plasmablasts, CD4+ and CD8+ T cells, regulatory T cells, γδ T cells, NK T cells, NK cells, and dendritic cells.

Key Findings

This optimized panel, based on OMIP-024, enables:

Comprehensive immune profiling from limited sample volumes.

Accurate antibody titration with NovoExpress software to ensure optimal resolution and minimal background.

In-depth analysis of NK and T cell activation and differentiation, critical for evaluating immune responses to vaccines and infections.


Figure 2. 18-color Pan-Leukocyte flow cytometry panel. PBMCs (50 μL) from a normal donor were stained with AViD, Fc Block, and an 18-color antibody panel, then analyzed on the NovoCyte Quanteon. (D) Activation status of CD4+ and CD8+ T cells was examined by the expression of CCR7, CD45RA, CD57, CD127, HLA-DR and CD38. 

Conclusion

The NovoCyte Quanteon empowers high-parameter, high-performance flow cytometry for broad and detailed immune surveillance. This 18-color panel demonstrates how modern cytometry can streamline complex analyses and expand our understanding of immune function.


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