Simplify Isolation of Monoclonal Gene-edited Colonies
Minimize guesswork. Maximize output.
CellRaft Technology simplifies the isolation of viable, gene-edited monoclonal colonies, overcoming key challenges in gene editing workflows.
How CellRaft Helps:
- Increases viable colony yield from fragile edited cells.
- Screens thousands of cells using a single consumable via automated image acquisition and software-driven analysis.
- Gently isolates cells without fluidics, ensuring higher viability.
Key Advantages
Supports single-cell growth
with shared media, unlike traditional single-cell-per-well methods.
Isolates colonies, not
individual cells, ensuring rapid expansion.
Can screen up to 40,000 cells
in a single process - equivalent to 416 96-well plates.
Workflow
Flask-Like Culture for Enhanced Cell Growth
Single cells are seeded on the CellRaft Array after introducing transgenic or CRISPR/CAS9 elements. This eliminates the need for trypsin, scraping, high-pressure fluidics, or limiting dilution. Thousands of CellRafts provide a shared media environment, ensuring cells remain physically separated yet not “alone.”
Image-based phenotypic screening
Using the CellRaft AIR® System, gene-edited cells can be imaged at
multiple time points to monitor colony formation. Knockout phenotypes, such as
GFP/RFP signal loss, can be tracked for precise phenotypic screening.
Cells transfected with a GFP-expessing plasmid were tracked for the growth of clonal colonies
Image-Based Single-Cell Analysis and Monoclonality Verification
CellRaft Cytometry™ software enables image-based verification of single cells to ensure monoclonality and track key parameters such as cell size, morphology, and gene expression over time.
With an intuitive interface, users can define target cell characteristics for software-guided CellRaft selection and automated isolation using the CellRaft AIR® System.
CellRaft Technology