Gene Editing Made Easier with CellRaft Technology
CellRaft Technology simplifies the isolation of viable, gene-edited monoclonal colonies, overcoming key challenges in gene editing workflows.
How CellRaft Helps:
- Increases viable colony yield from fragile edited cells.
- Screens thousands of cells using a single consumable via automated image acquisition and software-driven analysis.
- Gently isolates cells without fluidics, ensuring higher viability.
Key Advantages
- Supports single-cell growth with shared media, unlike traditional single-cell-per-well methods.
- Isolates colonies, not individual cells, ensuring rapid expansion.
- Can screen up to 40,000 cells in a single process - equivalent to 416 96-well plates.
Flask-Like Culture for Enhanced Cell Growth
Single cells are seeded on the CellRaft Array after introducing transgenic or CRISPR/CAS9 elements. This eliminates the need for trypsin, scraping, high-pressure fluidics, or limiting dilution. Thousands of CellRafts provide a shared media environment, ensuring cells remain physically separated yet not “alone.”
Using the CellRaft AIR® System, gene-edited cells can be imaged at
multiple time points to monitor colony formation. Knockout phenotypes, such as
GFP/RFP signal loss, can be tracked for precise phenotypic screening.
Cells transfected with a GFP-expessing plasmid were tracked for the growth of clonal colonies
Automated Identification & Isolation
CellRaft Cytometry™ software verifies monoclonality and analyzes key parameters—size, morphology, and gene expression. Users can define target colonies for software-guided selection and automated isolation via the CellRaft AIR System.