Flow Cytometry Antibodies 


Flow cytometry has become an essential tool for immunophenotyping, allowing the simultaneous immune profiling of blood, bone marrow, and dissociated tissues such as lymph nodes, spleen, and tumors. With well-characterized markers for immune cells, flow cytometry has significantly advanced immunology and immuno-oncology. High-sensitivity antibodies and cytometers enable the detection of rare cell populations, providing crucial insights into immune responses, disease mechanisms, and treatment efficacy. For example, immunophenotyping of human peripheral blood mononuclear cells (PBMCs) can reveal the proportions of immune cell populations, indicating immune responses or specific cellular changes. T cells, key players in adaptive immunity, are often analyzed using markers such as:


CD3

Pan-T cell marker

CD4

Helper % cell marker

CD8

Cytotoxic T cell marker

CD45

Hemotopoietic stem cell marker

CD56

Natural killer (NK) cell marker

CD19

B cell marker

CD25

Regulatory T cell (Treg) marker

CD38

Activation marker for immune cells

CD69

Early activation marker

HLA-DR

Activation marker for antigen-presenting cells

CD16

Fc receptor marker on NK cells and monocytes

CD11b

Myeloid and macrophage marker

CD14

Monocyte and macrophage marker

CD27

Memory B and T cell marker

CD86

Costimulatory molecule for antigen-presenting cells

CD137 (4-1BB)

T cell activation and costimulation marker

CD154 (CD40L)

T cell and antigen-presenting cell interaction marker​

CD5

T cell and B-1 cell marker

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