There are probably millions of PBMCs globally prepared from donor blood every day. The quality is however not only influenced by the selected separation media. It is very well known, that the composition also depends largely on the donor of the blood. The ratio between lymphocytes and monocytes can vary significantly between donors. Understanding these differences has a significant impact on the succes of the next steps performed with the PMBCs.

The overlay shows how dramatically the amount of lymphocytes and monocytes may vary from donor to donor. While the amount is balanced (red) in some cases, it may show a significant higher amount of monocytes (blue) or lymphocytes (yellow).