Organoids
From Stem Cells to Organoids
From Stem Cells to Organoids
The CERO 3D Incubator & Bioreactor provides an ideal environment for the cultivation and expansion of organoids from pluripotent or adult stem cells. It offers an efficient, standardizable way to generate and maintain high yields of homogeneous complex 3D models of various tissues and organs. Increase cellular, structural and functional complexity of organoids and to increase predictive capacity for in vivo response.
Benefits:
- Enhanced organoid growth and viability, also during long-term culture
- Improved reproducibility and physiological relevance
- Cost- and time-effective production of organoids
Gastric Organoids
In this study, CERO 3D provides an ideal system for the cultivation of gastric organoids. In this study, the organoids were composed of different cell types that are also common in stomach tissue. Furthermore, the organoids express markers of mature structures, such as gastric mucins MUC5AC and MUC6, trefoil factors, and pepsinogen.
The generation of gastric organoids is a crucial step in the study of Helicobacter pylori infection and gastric carcinogensis. The CERO 3D Incubator & Bioreactor allows the generation in a much more efficient, reproducible, physiological and cost efficient way compared to any other approach.
Scalable Production of Renal Cells from hPSCs
The CERO 3D Incubator and Bioreactor was used in developing a protocol for the large-scale production of renal proximal tubular epithelial cells (PTEC) from human pluripotent stem cells (hPSC). By utilizing suspension bioreactors for hPSC expansion and matrix-coated alginate beads for PTEC differentiation, this system supports the generation of cells crucial for functions like blood clearance, homeostasis, waste elimination, and substance reabsorption in pre-urine. These PTEC can be effectively used to model drug-induced nephrotoxicity, making them valuable for pharmaceutical research and development.
“Workflow overview of the differentiation of human iPSCs-derived hepatocytes in suspension culture (3D). The first step was to establish a robust 2D differentiation of HLCs from the initiation stage to the maturation stage. In the second step, the protocol was translated to a 3D cultivation regime in the suspension based bioreactor CERO 3D (OLS). Finally, hepatic organoids were cryopreserved at two differentiation time points (day 8 and 21) to assess the stock keeping efficiency. Different quality controls (QC) during differentiation and recovery after thawing show the quality of the hepatic organoids.”
Reference: Ngo et. al. (2022) Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system, Cell Prolif. 2022 Mar; 55(3): e13190.
Kidney Organoids
The CERO 3D system empowers the efficient generation of iPSC-derived kidney organoids, even with challenging cell lines. The system's direct inoculation capability streamlines workflows, eliminating the need for aggregation in ultra-low attachment plates.
iPSC-derived kidney organoids thriving in the CERO 3D.
Key features:
- Advanced Differentiation: Organoids exhibit mature structures, like proximal tubules (LTL positive), and express key markers such as WT1, indicating developing kidney structures and functionality
- Versatile Cultivation: Supports various cell lines, enabling diverse research applications.
- Enhanced Organoid Culture: The optimal environment leads to improved viability, reproducibility, and physiological relevance.
Courtesey: Prof. Schermer (Nephrolab Cologne, Kidney Research Center Cologne, KRCC), Germany
Your Versatile Platform for Cell Culture
Beyond stem cells and organoids, CERO 3D also supports the cultivation of:
- Punch biopsies
- Co-cultures
- Spheroids
- Single-cell suspensions
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