From Stem Cells to Organoids

From Stem Cells to Organoids

Download organoids report

   Kidney Organoids

The CERO 3D Incubator & Bioreactor is a revolutionary technology for production of organoids from pluripotent or adult stem cells. It offers an efficient, standardizable way to generate and maintain high yields of homogeneous organoids used as a tool in cancer research. Remarkably, the organoids have also a predictive capacity for in vivo response.

  • Reduced costs and time
  • Increase size & lifespan
  • Optimal nutrition & gas diffusion
  • Free floating, no shear forces
  • High homogeneity and yield
  • Multiplexing organoidogenesis

Increase cellular, structural and functional complexity of organoids.


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Efficient Generation of Organoids

CERO 3D provides an ideal environment for the cultivation and expansion of organoids derived from both iPSCs and adult stem cells, enabling researchers to create complex 3D models of various tissues and organs, including stomach organoids.

  • Benefits:
    • Enhanced organoid growth and viability
    • Improved reproducibility and physiological relevance
    • Cost-effective production of organoids

Gastric organoids (bright field) at day 7 and after splitting on day 22 expanding from small cysts that expand to bigger spheres

HE staining of Gastric organoids (2a) showing single layer of epithelia cells composed by the different cell types found in the stomach as seen in the PCR results (2b): expression of gastric mucins MUC5AC and MUC6, trefoil factors and pepsinogen, for instance. 

There are also stem cells markers like Lgr5 and Sox2. 


The generation of gastric organoids is a crucial step in the study of Helicobacter pylori infection and gastric carcinogensis. The CERO 3D Incubator & Bioreactor allows the generation in a much more efficient, reproducible, physiological and cost efficient way compared to any other approach.


Dr. Raquel Mejias-Luque, TUM, Germany
Scalable Production and Functional Differentiation of Renal Cells from Human Pluripotent Stem Cells

The CERO 3D Incubator and Bioreactor was used in developing a protocol for the large-scale production of renal proximal tubular epithelial cells (PTEC) from human pluripotent stem cells (hPSC). By utilizing suspension bioreactors for hPSC expansion and matrix-coated alginate beads for PTEC differentiation, this system supports the generation of cells crucial for functions like blood clearance, homeostasis, waste elimination, and substance reabsorption in pre-urine. These PTEC can be effectively used to model drug-induced nephrotoxicity, making them valuable for pharmaceutical research and development.  

“Workflow overview of the differentiation of human iPSCs-derived hepatocytes in suspension culture (3D). The first step was to establish a robust 2D differentiation of HLCs from the initiation stage to the maturation stage. In the second step, the protocol was translated to a 3D cultivation regime in the suspension based bioreactor CERO 3D (OLS). Finally, hepatic organoids were cryopreserved at two differentiation time points (day 8 and 21) to assess the stock keeping efficiency. Different quality controls (QC) during differentiation and recovery after thawing show the quality of the hepatic organoids.”

Reference: Ngo et. al. (2022) Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system, Cell Prolif. 2022 Mar; 55(3): e13190.

Kidney Organoids

The CERO 3D system empowers the efficient generation of iPSC-derived kidney organoids, even with challenging cell lines. The system's direct inoculation capability streamlines workflows, eliminating the need for aggregation in ultra-low attachment plates.

iPSC-derived kidney organoids thriving in the CERO 3D.

Courtesey: Prof. Schermer (Nephrolab Cologne, Kidney Research Center Cologne, KRCC), Germany

Key features:
  • Advanced Differentiation:  Organoids exhibit mature structures, like proximal tubules (LTL positive), and express key markers such as WT1, indicating developing kidney structures and functionality
  • Versatile Cultivation: Supports various cell lines, enabling diverse research applications.
  • Enhanced Organoid Culture: The optimal environment leads to improved viability, reproducibility, and physiological relevance.

 

Your Versatile Platform for Cell Culture

Beyond stem cells and organoids, CERO 3D also supports the cultivation of:

  • Punch biopsies
  • Co-cultures
  • Spheroids
  • Single-cell suspensions

 

Invite our experts and lift your research to the next stage with a demo 3D CERO Incubator and Bioreactor from OLS

​Join OLS and make your research reliable with our 3D CERO Incubator and Bioreactor with your lab-services as results.

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