We have succesfully been using CASY for 10 years now. With CASY we are able to carry out reproducible cell culture experiments even when working with highly aggregated cells. CASY allows us to consider cell concentration and aggregation when seeding and harvesting cells, as both are measured by CASY. Measuring accurately cell number, the aggregation factor and the cell volume, gives us the opportunity to express analyticial data as concentration per cell. Additionally and the molar amount of a substance within a cell can be calculated (used for example for metal uptake in 

Cytotoxicity Assays using CASY enable us to perform fast, simple and reliable assessment of anti-cancer drug regimens and the subsequent chemosensitivity of carcinoma cell lines. A major advantage of CASY is that debris and dead cells can be masked in the analysis by a simple cell line specific setup and only viable cells and their aggregates are considered. Howerver, as a complete cell status is recorded, also dead cells and debris data are available at any time.