[AL138] NCTC 109 is one of the chemically defined medium; originally formulated to establish and maintain a strain of mouse cells, L929. The medium supports growth of fibroblast-like and epithelial- like cells of both normal and malignant origin from mice, hamsters, monkeys and humans. AL138 contains sodium bicarbonate and co-enzyme A. It does not contain L-glutamine and HEPES.

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[AL139] Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. DMEM/Nutrient Mixture F-12 Ham with sodium bicarbonate, 15mM HEPES buffer and trace elements.

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[AL139A] Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. DMEM/Nutrient Mixture F-12 Ham is with Lglutamine, sodium bicarbonate, 15mM HEPES buffer and trace elements.

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[AL139G] HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. HiGlutaXL™ DMEM/Nutrient Mixture F-12 Ham with L-alanyl-L-glutamine, sodium bicarbonate, 15mM HEPES buffer and trace elements.

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[AL140] Dulbecco’s Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AL140 is DMEM/Nutrient Mixture F-12 Ham with 15mM HEPES buffer and sodium bicarbonate. It does not contain L-glutamine and trace elements.

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[AL140S] Dulbecco’s Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AL140S is DMEM/Nutrient Mixture F-12 Ham with 2.5mM L-Glutamine, 15mM HEPES buffer, 0.5mM Sodium pyruvate and 1.2gms per litre Sodium bicarbonate.

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[AL146] Ham’s Nutrient Mixture F12, Coon’s modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid. AL146 is Coon’s modification of Nutrient Mixture F-12 Ham without L-glutamine.

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[AL149] DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium with 1gm per litre of glucose, 25mM HEPES buffer, sodium bicarbonate and sodium pyruvate.

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[AL149A] Dulbecco's Modified Eagle Medium with L-glutamine, 1gm per litre of glucose, 25mM HEPES buffer, sodium bicarbonate and sodium pyruvate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.

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[AL150A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL150A is modified RPMI-1640 medium supplemented with L-glutamine and sodium bicarbonate. It does not contain glucose and HEPES.

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[AL151] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium with 4.5gms Glucose per litre, 25mM HEPES buffer, Sodium bicarbonate and Sodium pyruvate. It does not contain LGlutamine.

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[AL151A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium contains L-glutamine, 4.5gms glucose per litre, 25mM HEPES buffer, sodium bicarbonate and sodium pyruvate

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[AL154A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL154A contains Earle's salts, L-Glutamine, sodium bicarbonate and sodium pyruvate.

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[AL155A] Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. DMEM/Nutrient Mixture F-12 Ham with Lglutamine, sodium bicarbonate. It does not contain trace elements and HEPES buffer.

refrigerated goods (2°C - 8°C)

[AL155G] HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. HiGlutaXL™ DMEM/Nutrient Mixture F-12 Ham is with L-alanyl-L-glutamine and 2.438gms per litre sodium bicarbonate. It does not contain trace elements and HEPES buffer.

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[AL157] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL157 is modified RPMI-1640 medium supplemented with 25mM HEPES buffer and sodium bicarbonate. It does not contain L-glutamine.

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[AL160A] Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL070 contains L-glutamine and sodium bicarbonate. It does not contain HEPES buffer.

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[AL162A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL162A is modified RPMI-1640 medium supplemented with L-glutamine, 4.5gms glucose, 10mM HEPES buffer, sodium pyruvate and sodium bicarbonate.

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[AL162S] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL162S contains L-glutamine, 4.5gms per litre glucose, 10mM HEPES, 1.5gms per litre sodium bicarbonate and sodium pyruvate.

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[AL165A] Cytogenetic studies include metaphase and pro-metaphase studies carried out on lymphocytes to detect chromosomal aberrations associated with structural and numerical abnormalities. Lymphocytes come from normal peripheral blood and are mitotically inactive, hence have to be stimulated with a mitogen such as Phytohemagglutinin M(PHA M) or Phytohemagglutinin P(PHA-P). AL165A is composed of a basal medium RPMI 1640 and supplemented with L-glutamine, FBS, PHA-M, penicillin, streptomycin and sodium bicarbonate. It is a complete medium and does not require supplementation with any additional component.

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