During T cell activation, cell proliferation and increase in volume are two critical parameters that CASY tracks very precisely at the same time. In the example below, human CD4 T Cells were stimulated with anti-CD3/CD28 Dynabeads.

“Normal” cluster formation and cell size distribution during CD4 T cell stimulation. (Top) Cluster format in the initial phase of activation of human CD4 T cells. (Bottom) CD4 T cells increased their cell size from 8 μm in freshly isolated quiescent T cells (green line) to 10 μm after 24 h (blue line) and finally to 12 μm after 48 h of stimulation (red line).

Cluster formation and cell size development can change significantly under different metabolic conditions like the absence of glucose (A) or in the presence of Lactic acid (B).

Whatever the impact is, CASY will not only provide the exact cell number. You can also analyze very precisely changes in volume in each measurement.