[IM007] TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. IM007 contains L-glutamine and does not require supplementation with insect hemolymph. It is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines.
refrigerated goods (2°C - 8°C)